AB 22. Study of IL-1, IL-4 and TNFα polymorphisms in extrinsic and intrinsic asthma in a Greek population
Abstract

AB 22. Study of IL-1, IL-4 and TNFα polymorphisms in extrinsic and intrinsic asthma in a Greek population

Eirini Kontakioti1, Kalliopi Domvri1, Michalis Daniilidis2, Despina Papakosta1, Dimitrios Gioulekas1, Lazaros Sichletidis1, Konstantinos Porpodis1, Konstantinos Zarogoulidis1

1Asthma Clinic, Pulmonary Department, Aristotle University of Thessaloniki, George Papanikolaou Hospital, Exochi, Thessaloniki, Greece; 2Immunology Research Laboratory, 1st Department of Internal Medicine, Aristotle University of Thessaloniki ,AHEPA Hospital, Thessaloniki, Greece


Background: The aim of present study was the definition of the IL-1, IL-4 and TNFα polymorphisms in asthmatic patients and their association with allergy and bronchial hyperresponsiveness (BHR).
Materials and methods: Thirty patients with allergic asthma, mean age 35.2±14.51 years (Group A) and 22 patients with non-allergic asthma, mean age 47.1±16.3 years (Group B) were included in the study. All patients were recruited from the Asthma Clinic of Pulmonary Department, University of Thessaloniki. 21 healthy control subjects of mean age 36.6±10.5 years were also included (Group C). All patients were subjected to skin prick tests and metacholine challenge. DNA was extracted from the patients’ peripheral blood samples. Determination of IL1α -889(T/C), IL1β-511(T/C), IL1β+3962(C/T), IL1R1970(C/ T), IL1RA mspa111100(C/T), IL4-1098(T/G), IL4-590(C/T), IL4- 33(C/T), TNFα-308(G/A) and TNFα-238(G/A) polymorphisms were performed by PCR using the INVITROGEN kit (C: cytokine, T: thymine, G: Guanine και A: Adenine).
Results: Analysis of IL-1 polymorphisms and in particular IL-1β (+3962) showed statistically significant differences between Group A-B (P=0.029) and Group B-C (P=0.024) respectively. Furthermore, analysis for IL-1 receptor (IL1R) showed statistically significant differences between Group B and C, where C/T showed an increased frequency in Group B and C/C in Group C (P=0.003). Besides, analysis of IL-1 receptor antagonist (IL-1RA) in C/T and Τ/Τ genotypes showed increased frequencies in Group A and C respectively (P=0.03). Analysis of IL4 -1098 polymorphisms showed an increased frequency of guanine in Group A (P=0.036) when compared to Group C whereas for IL4-590, C/C genotype showed an increased frequency in Group C and C/T in Group A (P=0.003), suggesting possible association between thymine and risk of asthma development. Analysis of TNFα-238 in G/G and A/G genotypes showed statistically significant differences between the Group A-C, (P=0.008) and Group B-C (P=0.001). G/G showed an increased frequency in Group C and A/G in Groups A and B. Moreover C/T genotype for IL-4 showed positive association with high concentrations of methacholine (P=0.022) and subsequently low BHR. On the contrary, G/G genotype in TNFα gene were positively associated with lower concentrations of methacholine (P: 0.033) and high BHR.
Conclusions: IL-1, IL-4 and TNFα polymorphisms showed significant differences between patients with allergic and non-allergic asthma and healthy control subjects, which are possibly associated with risk of asthma development. BHR was associated with IL-1, IL-4 and TNFα polymorphisms.

Cite this abstract as: Kontakioti E, Domvri K, Daniilidis M, Papakosta D, Gioulekas D, Sichletidis L, Porpodis K, Zarogoulidis K. Study of IL-1, IL-4 and TNFα polymorphisms in extrinsic and intrinsic asthma in a Greek population. J Thorac Dis 2012;4(S1):AB22. DOI: 10.3978/j.issn.2072-1439.2012.s022

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