TY - JOUR AU - Zhong, Chang-Hao AU - Tong, Da AU - Zhou, Zi-Qing AU - Su, Zhu-Quan AU - Luo, Yu-Long AU - Xing, Jia AU - Bai, Ya-Li AU - Guo, Su- Jie AU - Li, Shi-Yue PY - 2018 TI - Performance evaluation of detecting circulating tumor cells and tumor cells in bronchoalveolar lavage fluid in diagnosis of peripheral lung cancer JF - Journal of Thoracic Disease; Vol 10, Supplement 7 (April 18, 2018): Journal of Thoracic Disease (Early Detection and Diagnosis of Lung Cancer) Y2 - 2018 KW - N2 - Background: To evaluate the diagnostic performances of detecting circulating tumor cells (CTCs) and tumor cells in bronchoalveolar lavage fluid (BALF) for peripheral lung cancer. Methods: A total of 247 patients with lung cancer and 70 cases with benign lung disease were recruited in this study. Peripheral blood and BALF samples were collected, in which the tumor cells were enriched by negative immunomagnetic selection and detected by fluorescence in situ hybridization (FISH) of chromosome enumeration probe 8 (CEP8). The levels of tumor-associated markers (e.g., CEA, CA125, and NSE) in peripheral blood plasma were measured by using electrochemiluminescence. Results: The numbers of CTCs detected in peripheral blood were significantly higher in patients with lung cancer than those with benign lung disease (5.78±0.57 vs . 1.13±0.39, Z=−8.64, P vs . 0.89±0.23, Z=−6.254, P 0.05). Detecting CTCs and tumor cells in BALF had similar areas under curves (AUC =0.871 and 0.963, respectively; P>0.05) in discriminating benign lesions from lung cancer (sensitivity 83.8% and 92.6%, specificity 86.5% and 99.9%, respectively), both of which were larger than those of NSE, CEA, and CA125 (AUC =0.564, 0.512 and 0.554, respectively; all P Conclusions: Detecting tumor cells in peripheral blood and BALF may sensitive to identify benign and malignant peripheral lung lesions and be of value for early diagnosis of lung cancer. UR - https://jtd.amegroups.org/article/view/18433