TY - JOUR AU - Shi, Hui AU - Shi, Jiahai AU - Zhang, Yudong AU - Guan, Chengqi AU - Zhu, Jun AU - Wang, Fei AU - Xu, Mingming AU - Ju, Qianqian AU - Fang, Shu AU - Jiang, Maorong PY - 2018 TI - Long non-coding RNA DANCR promotes cell proliferation, migration, invasion and resistance to apoptosis in esophageal cancer JF - Journal of Thoracic Disease; Vol 10, No 5 (May 31, 2018): Journal of Thoracic Disease Y2 - 2018 KW - N2 - Background: Long non-coding RNAs (lncRNAs) have important effects on the development and progression of multiple carcinomas. Our studies aimed to investigate the expression of lncRNA DANCR in esophageal squamous cell carcinoma (ESCC) tissues and paracancerous tissues, and to explore its effect on the cell biological characteristics of ESCC ECA109 cells. Methods: The expression of DANCR was detected by qRT-PCR in human ESCC tissues and paracancerous normal tissues in ESCC patients. Small interfering RNA (siRNA) was transfected to knock down the expression of DANCR and interference efficiency was analyzed by qRT-PCR in ECA109 cells. MTT, wound healing, Transwell, TUNEL and flow cytometry (FCM) assay was used to measure the influence of DANCR on proliferation, invasion, migration and apoptosis in ECA109 cells, respectively. Results: The expression of DANCR in ESCC tissues and ESCC cells was significantly higher compared with that in the adjacent normal tissues (P Conclusions: According to our research, the expression of DANCR was up-regulated in human ESCC tissues, and the important role that DANCR played in ESCC cells was similar to an oncogene. Therefore, silencing of lncRNA DANCR could have potentially beneficial effects on the prognostic and therapy for ESCC in the future. UR - https://jtd.amegroups.org/article/view/21366