%0 Journal Article %T Effect of normal saline flush injection into a bronchus on lung decellularization %A Yanagiya, Masahiro %A Kitano, Kentaro %A Yotsumoto, Takuma %A Takai, Daiya %A Asahina, Hiromichi %A Nagayama, Kazuhiro %A Nakajima, Jun %J Journal of Thoracic Disease %D 2019 %B 2019 %9 %! Effect of normal saline flush injection into a bronchus on lung decellularization %K %X Background: The aim of this study was to evaluate effect of normal saline flush injection into bronchus on creation of decellularized lung scaffolds. Methods: Pigs were used: 3 lung grafts for decellularization with pre-treatment of normal saline injection into a bronchus, 3 for decellularization without pre-treatment and 3 treated as normal controls. We compared the characteristics of lung scaffolds created by each method. Results: The pretreatment procedure significantly reduced the DNA content of lung grafts, suggesting effective removal of cellular components. However, this pretreatment also reduced the elastin contents of lung grafts. Conclusions: Considering this characteristic of saline pretreatment, we must continue to look for better methods to produce ideal decellularized lung grafts. %U https://jtd.amegroups.org/article/view/34046 %V 11 %N 12 %P 5321-5327 %@ 2077-6624