TY - JOUR AU - Yanagiya, Masahiro AU - Kitano, Kentaro AU - Yotsumoto, Takuma AU - Takai, Daiya AU - Asahina, Hiromichi AU - Nagayama, Kazuhiro AU - Nakajima, Jun PY - 2019 TI - Effect of normal saline flush injection into a bronchus on lung decellularization JF - Journal of Thoracic Disease; Vol 11, No 12 (December 31, 2019): Journal of Thoracic Disease Y2 - 2019 KW - N2 - Background: The aim of this study was to evaluate effect of normal saline flush injection into bronchus on creation of decellularized lung scaffolds. Methods: Pigs were used: 3 lung grafts for decellularization with pre-treatment of normal saline injection into a bronchus, 3 for decellularization without pre-treatment and 3 treated as normal controls. We compared the characteristics of lung scaffolds created by each method. Results: The pretreatment procedure significantly reduced the DNA content of lung grafts, suggesting effective removal of cellular components. However, this pretreatment also reduced the elastin contents of lung grafts. Conclusions: Considering this characteristic of saline pretreatment, we must continue to look for better methods to produce ideal decellularized lung grafts. UR - https://jtd.amegroups.org/article/view/34046