%0 Journal Article %T The expression of Nesprin-1 increased in aortic dissection: why? %A Yang, Wengang %A Wen, Dezhong %A Chen, Shaoxi %A Xue, Song %A Gu, Jianmin %A Dan, Jianggui %A Zheng, Hui %J Journal of Thoracic Disease %D 2019 %B 2019 %9 %! The expression of Nesprin-1 increased in aortic dissection: why? %K %X Background: To detect the expression of Nesprin-1 in aortic dissection (AD) in patients and to investigate the role of Nesprin-1 in the pathogenesis of AD in a mouse model. Methods: Blood and tissue specimens from AD patients were collected. The expression of Nesprin-1 in tissues from AD patients and non-AD patients with heart disease was studied by western blotting and quantitative real-time polymerase chain reaction (qRT-PCR). In addition, the expression and distribution of Nesprin-1 in AD and sham mice were compared in an induced AD mouse model, and detected by immunohistochemistry and qRT-PCR. Results: Immunoblotting and qRT-PCR both showed that the expression of Nesprin-1 was significantly higher in AD versus control patients. An animal model of AD was established by continuous injection of Ang II into ApoE −/− mice. The expression of Nesprin-1 in aortic tissue of AD mice was higher than that of sham- operated mice as determined by immunohistochemistry. qRT-PCR showed that Nesprin-1 gene expression in aorta of AD mice was higher than that of sham-operated mice. Conclusions: An increased expression of Nesprin-1 was associated with AD, and hence Nesprin-1 may be involved in the pathogenesis of ADs. Preliminary findings suggest that Nesprin-1 may be a therapeutic target for the treatment of AD. %U https://jtd.amegroups.org/article/view/34093 %V 11 %N 12 %P 4960-4965 %@ 2077-6624