Towards engineering integrated cardiac organoids: beating recorded

Towards engineering integrated cardiac organoids: beating recorded

Yu Shrike Zhang1, Cunjiang Yu2,3,4

1Division of Engineering in Medicine, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA, USA; 2Department of Mechanical Engineering, 3Department of Electrical and Computer Engineering, 4Program of Materials Science and Engineering, University of Houston, Houston, TX, USA

Correspondence to: Yu Shrike Zhang. Division of Engineering in Medicine, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA 02139, USA. Email:; Cunjiang Yu. Department of Mechanical Engineering, University of Houston, Houston, TX 77204, USA. Email:

Provenance: This is an invited Editorial commissioned by the Section Editor Kai Zhu (Department of Cardiac Surgery, Zhongshan Hospital Fudan University, Shanghai, China).

Comment on: Lind JU, Busbee TA, Valentine AD, et al. Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing. Nat Mater 2016. [Epub ahead of print].

Submitted Nov 23, 2016. Accepted for publication Nov 23, 2016.

doi: 10.21037/jtd.2016.12.37


Modeling human physiology in vitro is burgeoning as an exciting new field that requires coherent efforts encompassing multiple disciplines including engineering, biology, and medicine, among others (1). Originating from the concept of tissue engineering (2), yet with a distinct aim that is to construct models of human tissues and organs outside the body for improved biological, pharmaceutical, and environmental studies (3-6) rather than repairing them in vivo, these microphysiological systems have further undergone significant developments with the inclusion of the microfabrication and microfluidics technologies that conveniently bring in the beneficial complexity (4,7). While various microfabrication strategies allow us to engineer microscale tissue and organ units that possess shapes and architecture that mimic their in vivo counterparts (8-10), the ability to manipulate fluids at small scales leads to reproduction of the dynamic microenvironments indispensable for the functions of natural tissues and organs (1,4), both of which are otherwise not achievable using the conventional planar, static culture platforms. Of note, these individual microphysiological systems can be further linked together in such a way that the interconnected multi-unit platforms recapitulate the linkage of the various tissues and organs in their native arrangements, facilitating investigations of the intricate interactions among these different components in vitro (3-6).

To date, a variety of microphysiological systems have been developed that model their respective tissues and organs (and their disease states) of the human body, ranging from those mimicking the nervous system (11), the respiratory system (12,13), the digestive system (14,15), and the musculoskeletal system (16,17) to the cardiovascular system (18,19), covering essentially every single type of tissue and organ (4,5). Among all, the cardiac organoids have attracted increasing attention due to their critical roles in toxicology; for example, it is estimated that cardiotoxicity represents a major side effect of systemic drug toxicity—in the past 40 years 19% of drug recalls were likely due to cardiotoxicity (20).

A human heart contains four main chambers of two atria and two ventricles as well as valves and heart wall laden with specialized cell populations. The myocardium is responsible for contraction of the heart, which is primarily composed of cardiomyocytes densely aligned in parallel forming the bundled myocardial fibers and enhancing the contractile force to pump the blood throughout the vascular system of the body (21). Besides cardiomyocytes, approximately half of the cells in the heart are cardiac fibroblasts that produce the connective and elastic extracellular matrix (ECM) of the heart wall (21). Other important cell populations include the cardiac signal conduction system made of pacemakers cells and Purkinje fibers (21), as well as the endothelial cells that form the vasculature and supply nutrients to the cardiac cells (18).

Various approaches have been exploited to fabricate cardiac organoids and heart-on-a-chip devices that recapitulate the biology and physiology of a native heart. For instance, microengineering strategies that rely on the use of topographic cues would induce alignment of the cardiomyocytes that assume a similar structure of the native myocardium (22); bioprinting strategies have enabled the incorporation of vasculature into the cardiac organoids while maintaining the anisotropy of the cardiomyocytes (23); and the adoption of flexible substrates seeded with cardiomyocytes could lead to spontaneously actuating heart wall-like patterns modeling a part of a beating heart (24).

While significant efforts have been exerted on engineering functional cardiac organoids, ways to monitor their behaviors and responses have been limited. Common sensing strategies mainly rely on optical methods, either by imaging the contraction of the cardiac organoids (24,25), or by mapping the actions of the cardiomyocytes [e.g., Ca2+ flux following staining with fluorescence dyes (26)]. Besides, electrophysiological signals could also be measured by depositing microelectrode arrays onto the substrate on which the cardiomyocytes are housed (27). Nevertheless, these sensing elements have rarely been able to provide accurate, conformal measurements of the cardiac organoids as they typically would only represent projected collective behaviors (in the case of optical mapping) or planar signals (in the case of electrophysiology) of the model systems.

A recent publication by Lind et al., shed light on the existing dilemma, which for the first time provided an enabling strategy to fabricate fully integrated sensor-impregnated heart-on-a-chip device (Figure 1A,B) (28). Specifically, they used a three-dimensional (3D) multi-material extrusion printer with a multi-step procedure to generate the thin-film cantilever capable of deflecting by seeded cardiomyocytes, whereas the embedded conductive wires could directly measure the contractile signals during the deflections that would reflect the status of the cardiac organoids (Figure 1C): (I) printing the 0.5 µm-thick bottom release layer of dextran; (II) printing the 3 µm-thick cantilever base of thermoplastic polyurethane (TPU); (III) printing the 6.5 µm-thick strain gauge wires of TPU encapsulating 25 wt.% conductive carbon black nanoparticles; (IV) printing the 1.5 µm-thick TPU wire cover; (V) printing cardiomyocyte-guiding microfilaments of polydimethylsiloxane (PDMS); (VI) printing electrical leads and contact pads of silver:polyamide (Ag:PA); (VII) printing the well structure and insulation covers of PDMS; and (VIII) seeding the cardiomyocytes onto the printed cantilevers following curing of all the materials.

Figure 1 Fabrication of the sensor-impregnated heart-on-a-chip device. (A) schematic of the device principle: (I) contraction of an anisotropic engineered cardiac tissue (II) deflects a cantilever substrate, thereby stretching a soft strain gauge embedded in the cantilever, and (III) this generates a resistance change proportional to the contractile stress of the tissue; (B) the heart-on-chip device constructed from cardiomyocytes seeded on a set of fully printed device; (C) automated printing of the device on a 2×3 glass slide substrate in seven sequential steps. For each step, a corresponding still image from the printing procedure is shown. Cardiomyocytes were subsequently seeded onto each printed sensor-impregnated cantilever after it was fully cured. Adapted with permission from reference (28); copyright 2016 Nature Publishing Group. TPU, thermoplastic polyurethane; PDMS, polydimethylsiloxane.

Applying such a heart-on-a-chip system, the embedded strain gauge could conveniently measure the signals generated by each contracting cantilever via establishing a mechanical model that provided conversion between the changes in gauge resistance and those in cantilever radius/curvature, expressed as tissue twitch stress (σ), i.e., the difference between the systolic and diastolic stresses (Figure 2A). The contracting signals measured with the printed gauge on the cantilever were found to well correlate with the conventional optical tracking (Figure 2B), indicating the accuracy of the integrated sensing units and eliminating the needs for external instrumentation such as the use of a dedicated microscope. This sensor-impregnated heart-on-a-chip platform not only allowed for long-term culture and maturation of the human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs, Figure 2C) but more importantly, the real-time, in-line recording of their dose-dependent responses to pharmaceutical compounds (Figure 2D).

Figure 2 Monitoring the heart-on-a-chip device with impregnated strain gauge. (A) wide-field microscope images of cantilever bending upon tissue contraction: (I) minimum deflection corresponds to cardiac diastole and (II) peak deflection corresponding to systole. Right panel shows sketch of the mechanical model used to convert change in gauge resistance to stress generated by the tissue; (B) relative resistance changes (left axis) and corresponding calculated tissue twitch stress (right axis) recorded from spontaneously beating cantilever. The blue dotted line in the magnified view indicates stress determined independently by optical tracking of the cantilever radius of curvature; (C) representative traces of contractile twitch stress generated by the cantilevers populated with hiPS-CMs for up to 28 days of culture; (D) representative traces of twitch stress generated by a cantilever populated with hiPS-CMs when exposed to isoproterenol and corresponding dose-response curve, paced at 2 Hz. Adapted with permission from reference (28); copyright 2016 Nature Publishing Group.

This piece of work represents the first example of constructing a flexible organ-on-a-chip platform that is directly impregnated with sensing units for signal recording. The integration of the sensing elements is instrumental, allowing for seamless data collection and readouts of organoid status with no need of secondary devices. The conformal nature of the sensors further resulted in more accurate measurements that are less affected by the spatial movement of the 3D organoids than their projection-based or planar counterparts where the signals might be biased. Although currently the technology is still in its infant stage with limited complexity in the circuitry and types of signals that can be measured, its further optimization, development, and expansion into the combination with more sophisticated organoids potentially even diseased models [e.g., mitochondrial cardiomyopathy (29)], are envisioned in the near future.


Funding: YS Zhang acknowledges the National Cancer Institute of the National Institutes of Health Pathway to Independence Award (K99CA201603). C Yu acknowledges the financial support from University of Houston (Award for Excellence in Research, Scholarship or Creative Activity) and National Science Foundation (NSF-CMMI-1554499).


Conflicts of Interest: The authors have no conflicts of interest to declare.


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Cite this article as: Zhang YS, Yu C. Towards engineering integrated cardiac organoids: beating recorded. J Thorac Dis 2016;8(12):E1683-E1687. doi: 10.21037/jtd.2016.12.37