MicroRNA from tuberculosis RNA: A bioinformatics study
1Wiwanitkit House, Bangkhae, Bangkok, Thailand; 2Wiwanitkit House, Bangkhae, Bangkok Thailand; visiting professor, Hainan Medical
University, China; adjunct professor, Jospeh Ayobabalola university, Nigeria
The role of microRNA in the pathogenesis of pulmonary tuberculosis is the interesting topic in chest medicine at present.
Recently, it was proposed that the microRNA can be a useful biomarker for monitoring of pulmonary tuberculosis
and might be the important part in pathogenesis of disease. Here, the authors perform a bioinformatics study to assess
the microRNA within known tuberculosis RNA. The microRNA part can be detected and this can be important key
information in further study of the pathogenesis of pulmonary tuberculosis.
Pulmonary; tuberculosis; microRNA; RNA
J Thorac Dis 2012;4(3):296-297. DOI: 10.3978/j.issn.2072-1439.2012.05.08
Pulmonary tuberculosis is an important infectious disease.
This disease has been known for many years. However, there
is no success in control of this disease despite there is a long
history of BCG vaccine. The exact molecular pathogenesis of
pulmonary tuberculosis is not completely clarified and this
is still the myth in chest medicine. Based on the advanced
molecular laboratory at present, some new researches
report the importance of microRNA in the pathogenesis of
pulmonary tuberculosis. The microRNA could be detected in
blood of the patients with pulmonary tuberculosis (1
). Li et al.
reported that there is a genetic association between pulmonary
tuberculosis and SNPs within the corresponding miRNAs (2
Ma et al. found that microRNA miR-29 helped control innate
and adaptive immune responses to tuberculosis by targeting
). The detected microRNA is relating to the
clinical manifestation of disease and can be the biomarker for
The first success in clarification that microRNA takes role
in pulmonary tuberculosis pathogenesis was published in
). Hence, the knowledge on microRNA in pulmonary tuberculosis is very limited. Although it is no doubt that
microRNA might be detectable in blood of the patients, it
is still not known about its origin. The microRNA might
come from the host or pathogen and this is a topic for further
studies. Here, the authors perform a bioinformatics study to
assess the microRNA within known tuberculosis RNA. The
microRNA part can be detected and this can be important key
information in further study of the pathogenesis of pulmonary
Materials and methods
This is a bioinformatics study. The assessment of the RNA of
the Mycobacterium tuberculosis RNA was performed using a
standard computational bioinformatics method, Mireval (5
Briefly, this technique makes use of basic comparative structural
bioinformatics approach. The similarity comparison was done
based on structural clustering technique (6
). Blast was done
against mirBase (7
). Finally, an ab initio prediction method
was used for judging the detected microRNA (8
). In this work,
the RNA codon of strain AIIMS/LM/SS/TB-2132/06/SP
( JQ012998.1), which is the longest available RNA codon in
PubMed database at present were used as a primary template.
According to the study, there is a detected microRNA region
within the studied RNA. The position is at 500 to 580. The figure
showing this part is presented in Figure 1. The repeat analysis
showed the consistency of the results implying the precision and
accuracy of the analysis.
Figure 1. Showing the section that act as microRNA (picture directly generated by computational analysis based on Mireval).
MicroRNA is the specific kind of RNA that is the focus in
the study of pathogenesis of disease at present. Based on the
advancement in clinical molecular biology, the study of the
MicroRNA is possible. Also, it is accepted that the diagnostic
tool based on microRNA is the most sensitive diagnostic tool
at present. For pulmonary tuberculosis, Wu et al. noted that
microRNA expression profiling identifies, miR-155 and miR-
155* could be good potential diagnostic markers for active
). Another study by Fu et al. noted for the possible
use of miR-29 as biomarker for active pulmonary tuberculosis
). Also, a recent study noted that miR-21 has important role in
streptomycin treatment of pulmonary tuberculosis (10
The study on microRNA in pulmonary tuberculosis is useful.
The analysis of the RNA of pathogen is an interest aspect. Based
on this report, it can conclude that some detected microRNA
in the collected sample from the patient with tuberculosis come
from the RNA of the tubcerculosis pathogen. This means that
microRNA can come from either pathogen or patient. The
determination of the detected microRNA in this work can be the
new diagnostic approach. Conclusively, the finding of microRNA
can confirm that a part of microRNAs that play role in the
complex pathogenesis of pulmonary tuberculosis might come
from the pathogen. Further studies are required for assessment
the exact role of microRNAs in pathogenesis of disease.
Disclosure: The authors declare no conflict of interest.
- Guo W, Li JT, Pan X, et al. Candidate Mycobacterium tuberculosis genes
targeted by human microRNAs. Protein Cell 2010;1:419-21.
- Li D, Wang T, Song X, et al. Genetic study of two single nucleotide
polymorphisms within corresponding microRNAs and susceptibility to
tuberculosis in a Chinese Tibetan and Han population. Hum Immunol
- Ma F, Xu S, Liu X, et al. The microRNA miR-29 controls innate and
adaptive immune responses to intracellular bacterial infection by targeting
interferon-γ. Nat Immunol 2011;12:861-9.
- Fu Y, Yi Z, Wu X, et al. Circulating microRNAs in patients with active
pulmonary tuberculosis. J Clin Microbiol 2011;49:4246-51.
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- Ritchie W, Legendre M, Gautheret D. RNA stem-loops: to be or not to be
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- Griffiths-Jones S, Grocock RJ, van Dongen S, et al. miRBase: microRNA
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- Sewer A, Paul N, Landgraf P, et al. Identification of clustered microRNAs
using an ab initio prediction method. BMC Bioinformatics 2005;6:267.
- Wu J, Lu C, Diao N, et al. Analysis of microRNA expression profiling
identifies miR-155 and miR-155* as potential diagnostic markers for active
tuberculosis: a preliminary study. Hum Immunol 2012;73:31-7.
- Bose D, Jayaraj G, Suryawanshi H, et al. The tuberculosis drug streptomycin
as a potential cancer therapeutic: inhibition of miR-21 function by directly
targeting its precursor. Angew Chem Int Ed Engl 2012;51:1019-23.
Cite this article as: Wiwanitkit S, Wiwanitkit V. MicroRNA from
tuberculosis RNA: A bioinformatics study. J Thorac Dis 2012;4(3):296-297.